ENTEROBACTER GERGOVIAE PDF

Enterobacter gergoviae is a recurrent contaminant of cosmetic and hygiene products. To understand how this bacterium adapts to biocides, we studied Ent. Abstract. Objectives: In order to characterize the mechanism involved in parabens resistance, we studied 13 Enterobacter gergoviae collected. Clin Microbiol Infect. Sep;8(9) Natural antibiotic susceptibility of Enterobacter amnigenus, Enterobacter cancerogenus, Enterobacter gergoviae.

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Phenotypic changes contributing to Enterobacter gergoviae biocide resistance. Enterobacter gergoviae is a recurrent contaminant of cosmetic and hygiene products. To understand how this bacterium adapts to biocides, we studied Ent. They were compared with others also isolated from contaminated gergkviae. Phenotypic differences were noted and these included changes in the bacterial envelope and flagella along with differences in motility, and biofilm growth rates.

No differential phenotypes were noted for carbon source utilisation. Enterobacter gergoviae demonstrated a diverse response to both of these preservatives contained in cosmetic preparations, depending on their concentrations.

Interestingly, this adaptive response is associated with modifications of filament structure-related proteins contributing to increase the organism motility and the production of biofilm. Recurrent contaminations of cosmetics products gergovie Ent.

We demonstrated that bacteria response is associated to various mechanisms represented by expression of external appendages pili gerglviae fimbriae that control cell motility and biofilm formation and evolving as the concentration of biocides adaptation increased.

Such mechanisms which are not chemical specific can also promote a cross-resistance to other biocidal agents. Enterohacter characterization of Ent. Enterobacter gergoviae adaptation to preservatives commonly used in cosmetic industry.

The aim of this study was to obtain a better understanding regarding the origin of recurrent contamination by Enterobacter gergoviae in diverse cosmetic formula. We studied 65 isolates collected from various sources clinical, food, cosmetics. Evaluation of susceptibility to preservatives currently used in cosmetics for a representative panel of collection strains was measured.

Preservative efficacy was evaluated by minimum inhibitory concentrations and minimum bactericidal concentrations MBCs. Eighty per cent of isolates was unrelated. Association of preservatives showed in rare case additive effects, and no synergic effects were observed.

Most of the cosmetic formulations are contaminated with unrelated E. Maximum allowed concentrations for sodium benzoate are inefficient to limit proliferation and control adaptability to this bacterium in cosmetic products. Efflux mechanisms should be involved in methylisothiazolinone-chloromethylisothiazolinone and triclosan adaptation. Enterobacter gergoviae membrane modifications are involved in the adaptive response to preservatives used in cosmetic industry.

The objective of this study was to understand the adaptive mechanisms in Enterobacter gergoviae which are involved in recurrent contaminations in cosmetic products that are incorporated with preservatives.

It included a series of Ent. The other series was of Ent. Enrerobacter evaluated the outer membrane protein modifications and efflux mechanisms activities responsible for the resistant trait via immunoblotting assays. Additionally, for understanding the efflux activity real-time efflux, experiments were performed.

A cross-insusceptibility between preservatives and some disinfectants was observed in MIT-CMIT-resistant derivative isolates, but antibiotics susceptibility was not altered.

Furthermore, an array of real-time efflux assays indicated different activity levels while no variations were detected in porins and AcrAB-TolC pumps production. A similar profile was also observed among strains isolated from cosmetic products.

They might be involved in recurrent episodes of contaminations occurring in the cosmetic production. Constitutive expression of the nifA gene activates associative nitrogen fixation of Enterobacter gergoviaean opportunistic endophytic diazotroph. This study was undertaken to investigate whether a nitrogen-fixing bacterium Enterobacter gergoviae gsrgoviae, which was isolated from surface-sterilized maize Zea mays L.

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Maize seedlings grown in an agar medium were inoculated with Ent. Root colonization and expression of the dinitrogenase reductase gene nifH by Ent.

Enterobacter gergoviae is an opportunistic endophyte because it can live in soil and colonize grrgoviae maize roots in the gnotobiotic agar culture. In agreement with previous 15 N-dilution evidence that Ent. This study and our previous studies suggest that manipulation of the promoter of the nifA gene in a nitrogen-fixing bacterium having a high colonization competence is a practical and promising approach to achieve a stable associative nitrogen fixation for cereals.

Pneumonia due to Enterobacter cancerogenus infection.

Enterobacter cancerogenus formerly known as CDC Enteric Group 19; synonym with Enterobacter taylorae has rarely been associated with human infections, and little is known regarding the epidemiology and clinical significance of this organism.

We describe a community-acquired pneumonia case in a year-old female due to E. The clinical case suggests that E. We describe the interspecies transmission of the plasmid-mediated blaKPC-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. The strains showed identical plasmids.

Since intense horizontal exchanges among bacteria can occur in the gut, clinicians should be aware that patients colonized by carbapenem-resistant K. Comparative in vitro activities of ciprofloxacin, clinafloxacin, gatifloxacin, levofloxacin, moxifloxacin, and trovafloxacin against Klebsiella pneumoniaeKlebsiella oxytoca, Enterobacter cloacae, and Enterobacter aerogenes clinical isolates with alterations in GyrA and ParC proteins.

The in vitro activities of ciprofloxacin, clinafloxacin, gatifloxacin, levofloxacin, moxifloxacin, and trovafloxacin were tested against 72 ciprofloxacin-resistant and 28 ciprofloxacin-susceptible isolates of Klebsiella pneumoniaeKlebsiella oxytoca, Enterobacter cloacae, and Enterobacter aerogenes. Irrespective of the alterations in GyrA and ParC proteins, clinafloxacin exhibited greater activity than all other fluoroquinolones tested against K.

The taxonomy of Enterobacter has a complicated history, with several species transferred to and from this genus. Classification of strains is difficult owing to its polyphyletic nature, based on 16S rRNA gene sequences. It has been previously acknowledged that Enterobacter contains species which should be transferred to other genera.

In an attempt to resolve the taxonomy of EnterobacterMLSA based on partial sequencing of protein-encoding genes gyrB, rpoB, infB and atpD was performed on the type strains and reference strains of EnterobacterCronobacter and Serratia species, as well as members of the closely related genera Citrobacter, Klebsiella, Kluyvera, Leclercia, Mangrovibacter, Raoultella and Yokenella.

Phylogenetic analyses of the concatenated nucleotide sequences revealed that Enterobacter can be divided into five strongly supported MLSA groups, suggesting that the species should be reclassified into five different genera. Further support for this was provided by a concatenated amino acid tree, phenotypic characteristics and fatty acid profiles, enabling differentiation of the MLSA groups. Three novel genera are proposed: Additionally, the novel epithet Cronobacter zurichensis nom.

Physiological and molecular characteristics of carbapenem resistance in Klebsiella pneumoniae and Enterobacter aerogenes. Bacterial resistance is a growing concern in the nosocomial environment in which Klebsiella pneumoniae and Enterobacter aerogenes play an important role due to their opportunism and carbapenemase-production.

This work aimed to evaluate physiological and molecular characteristics of carbapenem-resistant K. In total, 42 carbapenem-resistant bacteria isolated from clinical specimens were included 21 K. Antimicrobial susceptibility and biocide tolerance patterns, hemolytic activity, tolerance to oxidative stress, and aggregative ability were assessed.

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Compared to drug-sensitive strains, carbapenem-resistant K. The genetic markers blaKPC Among the carbapenem-resistant E. Results suggest that carbapenemase-producing enterobacteria might show peculiar characteristics regarding their physiology associated with their environmental persistency, virulence, and multidrug resistance.

The observed phenomenon may have implications not only for antimicrobial chemotherapy, but also for the prognosis of infectious diseases and infection control.

Enterobacter – Wikipedia

In this study, outer membrane proteins OMPs of colistin-resistant Klebsiella vergoviae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. OMP analysis of each strain was performed by microchip method. OmpC and OmpW were gergovviae in the colistin-susceptible E. This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains.

The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae. Severe community-acquired Enterobacter pneumonia: Fnterobacter primary aim was to describe them as few data are available on EnCAP.

A comparison with CAP due to common and typical bacteria was performed. A univariate and multivariate logistic regression analysis was performed to determine factors independently associated with ENCAP. Their outcome was also compared. Eighty-five isolates of Klebsiella pneumoniae and Enterobacter spp.

Enterobacter gergoviae

This is a Yersinia typical gene involved in the synthesis of siderophore yersiniabactin. DNA sequencing confirmed the identity of irp2 gene in five K. To our knowledge in the current literature, this is the first report of the irp2 gene in E. Additionally, the analyses of nucleotide and amino acid sequences suggest the irp2 genes derived from isolates used in this study are more closely related to that of Yersinia pestis P. CE than to that of Yersinia enterocolitica These data demonstrated that K.

Clinical isolates of Enteeobacter pneumoniae and Enterobacter spp. Isolates bearing these genes emerged in the mids, coinciding with the ebterobacter of a rapid increase in fluoroquinolone resistance. Sixty percent of these isolates were ciprofloxacin susceptible by CLSI breakpoints. The discrimination between both species, which is routinely based on biochemical characteristics, is generally accepted to be straightforward. Here we report that genotypically unrelated strains of E. Results Ten clinical isolates, identified as K.

Misidentification also occurred when using the automated identification systems Vitek 2 and Phoenix, and was due to delayed positivity for ornithine decarboxylase and motility.

Subculture and prolonged incubation resulted in positive results for ornithine decarboxylase and for motility. The phenomenon may gergovise widespread, since it was shown to occur among genotypically unrelated strains from different hospitals and different isolation dates.

References

In several instances, the use of genotypic techniques like tDNA-PCR may circumvent problems of phenotypic identification. Occurrence of efflux mechanism and cephalosporinase variant in a population of Enterobacter aerogenes and Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases.

We investigated the occurrence of multidrug resistance in 44 Enterobacter aerogenes and Klebsiella pneumoniae clinical isolates. Efflux was involved in resistance in Enteeobacter. AcrA-TolC overproduction in E.